These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Reagentless uric acid biosensor based on Ni microdiscs-loaded NiO thin film matrix.
    Author: Arora K, Tomar M, Gupta V.
    Journal: Analyst; 2014 Sep 21; 139(18):4606-12. PubMed ID: 25046556.
    Abstract:
    The development of a noninvasive test for uric acid has been the holy grail of uric acid detection research over the last decade. In the present work, a novel matrix comprising of a NiO thin film (a biocompatible material) loaded with Ni microdiscs was prepared on an ITO-coated glass substrate (Ni/NiO/ITO) with the help of RF sputtering for the reagentless detection of uric acid. The bioelectrode was fabricated by immobilizing uricase using a physical adsorption technique on the surface of the Ni/NiO/ITO electrode. The prepared matrix was found to be efficient in sensing biological processes occurring on the surface of the bioelectrode (Ur/Ni/NiO/ITO) in the presence of the analyte (uric acid) to obtain an electronic output. The biosensor exhibits a high sensitivity (431.09 μA mM(-1)), low Km value (0.15 mM), high apparent enzyme activity (5.07 × 10(-2) units per cm(2)), high shelf life (20 weeks) and good selectivity for the detection of uric acid over a wide concentration range (0.05 mM to 1 mM) without any external mediator in the PBS buffer. The obtained results are encouraging for the realization of a reagentless uric acid biosensor with efficient sensing response characteristics.
    [Abstract] [Full Text] [Related] [New Search]