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  • Title: A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro.
    Author: Bober MA, Milco LA, Miller RB, Mount M, Wicks B, Kurth MJ.
    Journal: Toxicon; 1989; 27(9):1059-64. PubMed ID: 2508272.
    Abstract:
    Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 micrograms/microliters) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.
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