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  • Title: Expression of the trpC gene from Penicillium chrysogenum in Aspergillus nidulans.
    Author: Picknett TM, Saunders G, Holt G.
    Journal: Biotechnol Appl Biochem; 1989 Oct; 11(5):464-70. PubMed ID: 2508698.
    Abstract:
    The heterologous expression in Aspergillus nidulans of a gene involved in tryptophan biosynthesis from Penicillium chrysogenum is described. With the chimeric plasmid pPC-31, which carries the cloned trpC gene, approximately 10-40 "stable" transformants per microgram of DNA were obtained, with selection for complementation of the mutant allele. This frequency was increased 10-fold by the insertion of the ans1 fragment into the transformation vector. Southern hybridization analysis revealed that transformation occurred as a consequence of the integration of vector sequences into the host chromosome at a variety of sites within the genome.
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