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  • Title: Changes in mRNA levels of poly(ADP-ribose) polymerase during activation of human lymphocytes.
    Author: McNerney R, Tavasolli M, Shall S, Brazinski A, Johnstone A.
    Journal: Biochim Biophys Acta; 1989 Nov 02; 1009(2):185-7. PubMed ID: 2508750.
    Abstract:
    The level of mRNA encoding the nuclear enzyme poly(ADP-ribose) polymerase (ADP-ribosyltransferase, EC 2.4.2.30) was found to be very low in quiescent human lymphocytes and to increase at least 10-fold between 1 and 2 dyas after stimulation with the mitogen phytohaemagglutinin, staying high for several days thereafter. This increase was inhibited by 3-methoxybenzamide (a competitive inhibitor of poly(ADP-ribose) polymerase) but was not affected significantly by aphidicolin. Incubation of activated cells with cycloheximide for 2 h increased the expression slightly. These data demonstrate that, during lymphocyte activation, the level of mRNA of the poly(ADP-ribose) polymerase gene correlates with, and hence is presumably responsible for, the increase in poly(ADP-ribose) polymerase protein detectable by enzyme assay or immunochemistry.
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