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  • Title: [Study on HPLC fingerprint of Ganershu intermediate and determination of three active components].
    Author: Ge SB, Hou ZS, Yan GJ, Pan JH.
    Journal: Zhong Yao Cai; 2013 Dec; 36(12):2034-8. PubMed ID: 25090692.
    Abstract:
    OBJECTIVE: To establish the fingerprint analysis method of Ganershu intermediate by HPLC. METHODS: An analysis was performed on a sunFire C18 (4. 6 mm x 250 mm, 5 microm) column with acetonitrile-0.1% phosphate aqueous as the mobile phase by gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 320 nm and detection time was 80 min. The column temperature was 35 degrees C. In the recorded chromatogram of Ganershu intermediate, neohesperidin was used as reference substance, and RSD of the relative retention time and the relative peak areas of all peaks compared with its peak were measured. The similarity of 10 batches of Ganershu intermediate was appraised by the similarity evaluation system. Using the external standard method, the contents of chlorogenic acid, neohesperidin and naringin were determined in 10 batches of intermediate. RESULTS: Tweenty-three peaks were separated on HPLC fingerprint in Ganershu intermediate, degree of similarity of fingerprint for ten batches of Ganershu intermediate were greater than 0.90. Three compounds' contents were almost the same in each batch of intermediate sample. CONCLUSION: The method is stable, accurate, reliable and can be used as a quality control for Ganershu intermediate and sustained-release capsules.
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