These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of four kinds of purines in deer fetus soft capsule by HPLC].
    Author: Song JP, Zhu M.
    Journal: Zhong Yao Cai; 2014 Jan; 37(1):19-21. PubMed ID: 25090696.
    Abstract:
    OBJECTIVE: To establish an HPLC method for simultaneous determination of four kinds of purines in deer fetus soft capsule. METHODS: Four kinds of purines were detected and determined by HPLC. The mobile phase was 0.02 mol/L KH2PO4 (containing 1 mmol/L heptane sulfonic acid sodium, pH = 3.8)-methanol = 97:3. Detection wavelength was 254 nm and flow rate was 1.5 ml min. The linear relationship of four kinds of purines was as follows: hypoxanthine: Y1 = 83695X1 + 355 (r1 = 0.9998), with the linear range 0.040-0.667 mg/mL; xanthine: Y2 = 50638X2 + 39 (r2 = 0.9989), with the linear range 0.008-0.119 mg/mL; guanine: Y3 = 30269X3-9562 (r3 = 0.9924), with the linear range 0.018 - 0.279 mg/mL; adenine: Y4 = 38975X4-8671 (r4 = 0.9989), with the linear range 0.027-0.399 mg/mL The average sample recovery rate of hypoxanthine, xanthine, guanine and adenine were 98.1%, 98.6%, 98.0% and 97.5%, with RSD 1.0%, 0.4%, 0.8% and 0.6%, respectively. RESULTS: The content of hypoxanthine, xanthine, guanine and adenine in 3 lots of deer fetus soft capsule were 116.5-132.0 microg/capsule, 21.2-23.0 microg/capsule, 48.6-54.3 microg/capsule and 68.9-75.2 microg/capsule, respectively. CONCLUSIONS: This method is simple,accurate and reproducible, which provides a basis for quality control of purines in deer fetus soft capsule.
    [Abstract] [Full Text] [Related] [New Search]