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  • Title: Identification of GTP-binding proteins in Fasciola hepatica and Schistosoma mansoni by immunoblotting.
    Author: Mansour JM, Mansour TE.
    Journal: Mol Biochem Parasitol; 1989 Aug; 36(1):11-8. PubMed ID: 2509906.
    Abstract:
    Both the liver fluke Fasciola hepatica and the blood fluke Schistosoma mansoni have GTP-binding proteins which are part of the trans-membrane signalling system. These proteins require GTP in order to interact with the catalytic subunit of adenylate cyclase. The technique of immunoblotting was used in order to distinguish the GTP-binding proteins Gs, Gi, and Go. Immunoblotting was carried out using antisera prepared against peptides deduced from bovine cDNA clones specific for alpha or beta subunits of known G proteins. A 41-kDa Gs alpha has been identified in S. mansoni and a 42.5-kDa Gs alpha in F. hepatica. A 41-kDa Go alpha was found in both parasites. A 36-kDa G beta was identified in both parasites using antiserum made against bovine transducin.
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