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  • Title: [Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro].
    Author: Zhang XB, Liu TL, Zhang X, Tan RJ, Ge HL, Huang YL.
    Journal: Shanghai Kou Qiang Yi Xue; 2014 Jun; 23(3):280-4. PubMed ID: 25102867.
    Abstract:
    PURPOSE: To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs. METHODS: BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10⁻⁵, 1×10⁻⁶, 1×10⁻⁷, 1×10⁻⁸ and 1×10⁻⁹ mol/L) were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase (ALP). The formation of nodules of calcium was detected by von Kossa staining. The data was analyzed with SPSS20.0 software package. RESULTS: The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10-6 mol/L. Calcium nodule (von Kossa) staining was positive. CONCLUSIONS: Naringin-induced bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10-6 mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.
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