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  • Title: [Studies on endotoxin of Leptospira. V. The effect of EDTA-Na on the release of the LPS of Leptospira interrogans serovar lai].
    Author: Wu SH, Jiang SX, Wang HQ, Nie DK, Zhu GF.
    Journal: Wei Sheng Wu Xue Bao; 1989 Oct; 29(5):390-3. PubMed ID: 2511666.
    Abstract:
    The yield of leptospiral LPS was able to increase 1-2 times when the cells were treated with EDTA-Na before extracting with hot phenal-water method. The colours and solubility of L-LPS were improved. When the leptospiral cells were stored for a long period of time, and treated by EDTA-Na, the phase distribution of the LPS changed, i.e. they returned from water phase into phenol phase. Such change was related to the quantity of both the saccharide and lipid.
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