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  • Title: Dissolution-enhanced luminescent bioassay based on inorganic lanthanide nanoparticles.
    Author: Zhou S, Zheng W, Chen Z, Tu D, Liu Y, Ma E, Li R, Zhu H, Huang M, Chen X.
    Journal: Angew Chem Int Ed Engl; 2014 Nov 10; 53(46):12498-502. PubMed ID: 25131425.
    Abstract:
    Conventional dissociation-enhanced lanthanide fluoroimmunoassays (DELFIA) using molecular probes suffer from a low labeling ratio of lanthanide ions (Ln(3+) ) per biomolecule. Herein, we develop a unique bioassay based on the dissolution-enhanced luminescence of inorganic lanthanide nanoparticles (NPs). As a result of the highly concentrated Ln(3+)  ions in a single Ln(3+)  NP, an extremely high Ln(3+)  labeling ratio can be achieved, which amplifies significantly the luminescence signal and thus improves the detection sensitivity compared to DELFIA. Utilizing sub-10 nm NaEuF4  NPs as dissolution-enhanced luminescent nanoprobes, we demonstrate the successful in vitro detection of carcinoembryonic antigen (CEA, an important tumor marker) in human serum samples with a record-low detection limit of 0.1 pg mL(-1) (0.5 fM). This value is an improvement of approximately 3 orders of magnitude relative to that of DELFIA. The dissolution-enhanced luminescent bioassay shows great promise in versatile bioapplications, such as ultrasensitive and multiplexed in vitro detection of disease markers in clinical diagnosis.
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