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  • Title: Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes.
    Author: Nomura H, Hachisuka H, Higuchi M, Sasai Y.
    Journal: Acta Histochem; 1989; 87(1):59-62. PubMed ID: 2513698.
    Abstract:
    Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes.
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