These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of uraemic status on immunofluorescence and enzyme-linked immunosorbent assays for detection of human herpesvirus type 8 antibodies.
    Author: Tsai JP, Lin MN, Su CC.
    Journal: Pathology; 2014 Oct; 46(6):566-8. PubMed ID: 25158811.
    Abstract:
    Our previous study showed that human herpesvirus type 8 (HHV-8) seroprevalence was significantly higher in patients with end-stage renal disease (ESRD) immediately after haemodialysis than in healthy controls based on an immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA). However, other studies indicated that ESRD patients and healthy controls had similar HHV-8 seroprevalence. This study aimed to investigate whether this discrepancy is due to the effect of uraemic status.Plasma samples from 162 ESRD patients, taken immediately before and after haemodialysis, and 162 age and sex matched healthy controls were analysed for HHV-8 antibodies using both IFA and ELISA.HHV-8 seropositivities based on IFA and ELISA, both before and after haemodialysis, were significantly greater in ESRD patients than in healthy controls (p < 0.008 for all comparisons). The seropositivities and antibody titres of ESRD patients obtained with IFA were similar before and after haemodialysis. Seropositivities based on ELISA were identical before and after haemodialysis. The seropositivities obtained with the IFA markedly exceeded those with ELISA in each group of subjects (p < 0.0001 for all comparisons).Uraemic status did not significantly affect the IFA or ELISA results for HHV-8 antibodies.
    [Abstract] [Full Text] [Related] [New Search]