These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma].
    Author: Chubachi A.
    Journal: Rinsho Ketsueki; 1989 Dec; 30(12):2134-40. PubMed ID: 2516139.
    Abstract:
    The objective of this study is to demonstrate the diagnostic usefulness of flow cytometric analysis of surface immunoglobulin (S-Ig) light chains and monoclonal antibodies (MoAbs) in B-cell non-Hodgkin's lymphoma. For this purpose, the biopsied specimen (lymph nodes, tonsils, and spleens etc) cell suspensions from 44 patients were studied to detect the expression of S-Ig and several antigens recognized by MoAbs. A tumor was considered B-lineage if expression of pan B antigens and/or monoclonal light chains (S-Ig and/or cytoplasmic Ig) was detected in the absence of pan T antigens. Monoclonal expression of S-Ig light chain was detectable in 37 of 44 (84%) patients with B-cell lymphoma. In two of three cases having polyclonal S-Ig light chain, monoclonal C-Ig light chain was detected in large cells on paraffin-embedded tissues with immunohistochemical technique. Pan B antigens were strongly positive in four cases with negative S-Ig light chain. In diffuse large cell lymphoma, expression of CD11b was restricted to non-cleaved cell type (DLN). CD10 was favorably expressed by non-cleaved cell type and to a lesser extent, by immunoblastic histology (LI), but was not detected on cases with cleaved cell type (DLC). Furthermore, relatively uniform expression of S-IgM was seen in DLC subgroup, and on the contrary, heterogenous S-Ig was shown in other histology groups (DLN, LI). Flow cytometry provides a rapid, objective technology to confirm the immunological diagnosis of B-cell non-Hodgkin's lymphoma.
    [Abstract] [Full Text] [Related] [New Search]