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  • Title: Label-free photoelectrochemical immunosensor for sensitive detection of Ochratoxin A.
    Author: Yang J, Gao P, Liu Y, Li R, Ma H, Du B, Wei Q.
    Journal: Biosens Bioelectron; 2015 Feb 15; 64():13-8. PubMed ID: 25173733.
    Abstract:
    A general label-free photoelectrochemical (PEC) platform was manufactured by assembly of CdSe nanoparticles (NPs) sensitized anatase TiO2-functionalized electrode via layer-by-layer (LBL) strategy. CdSe NPs were assembled on anatase TiO2-functionalized electrode through dentate binding of TiO2 NPs to -COOH groups. Ascorbic acid (AA) was used as an efficient electron donor for scavenging photogenerated holes under visible-light irradiation. The photocurrent response of the CdSe NPs modified electrode was significantly enhanced as a result of the band alignment of CdSe and TiO2 in electrolyte. Ochratoxin A (OTA), as model analyte, was employed to investigate the performance of the PEC platform. Antibodies of OTA were immobilized on CdSe sensitized electrode by using the classic 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride coupling reactions between -COOH groups on the surfaces of CdSe NPs and -NH2 groups of the antibody. Under the optimized conditions, the photocurrent was proportional to OTA concentration range from 10pg/mL to 50ng/mL with detection limit of 2.0pg/mL. The employed PEC platform established a simple, fast and inexpensive strategy for fabrication of label-free biosensor, which might be widely applied in bioanalysis and biosensing in the future.
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