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  • Title: ¹³C-metabolic flux analysis for Escherichia coli.
    Author: Matsuoka Y, Shimizu K.
    Journal: Methods Mol Biol; 2014; 1191():261-89. PubMed ID: 25178796.
    Abstract:
    (13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression, enzyme activities, and metabolite concentrations as well the result of (13)C-MFA.
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