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  • Title: SEAL by NMR: glyco-based selenium-labeled affinity ligands detected by NMR spectroscopy.
    Author: Hamark C, Landström J, Widmalm G.
    Journal: Chemistry; 2014 Oct 20; 20(43):13905-8. PubMed ID: 25196366.
    Abstract:
    We report a method for the screening of interactions between proteins and selenium-labeled carbohydrate ligands. SEAL by NMR is demonstrated with selenoglycosides binding to lectins where the selenium nucleus serves as an NMR-active handle and reports on binding through (77)Se NMR spectroscopy. In terms of overall sensitivity, this nucleus is comparable to (13)C NMR, while the NMR spectral width is ten times larger, yielding little overlap in (77)Se NMR spectroscopy, even for similar compounds. The studied ligands are singly selenated bioisosteres of methyl glycosides for which straightforward preparation methods are at hand and libraries can readily be generated. The strength of the approach lies in its simplicity, sensitivity to binding events, the tolerance to additives and the possibility of having several ligands in the assay. This study extends the increasing potential of selenium in structure biology and medicinal chemistry. We anticipate that SEAL by NMR will be a beneficial tool for the development of selenium-based bioactive compounds, such as glycomimetic drug candidates.
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