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  • Title: [Umbilical cord blood-derived mesenchymal stem cells inhibit proliferation of peripheral blood lymphocytes].
    Author: Xu Z, Sheng L, Ouyang G.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2014 Sep; 30(9):968-71. PubMed ID: 25200160.
    Abstract:
    OBJECTIVE: To study the effect of umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) on the proliferation of adult peripheral blood lymphocytes. METHODS: MSCs were isolated and amplified from human UCB and identified by detecting the surface markers and the ability of differentiation into osteoblasts and adipocytes. Lymphocytes were stimulated with phytohemagglutinin (PHA) and co-cultured with UCBMSCs, or cultured with UCBMSCs in non-contact Transwell(TM) system. Two culture systems were both divided into negative control group (lymphocytes were cultured alone), positive control group (lymphocytes were stimulated with PHA), and experimental group (lymphocytes were cultured with UCBMSCs and stimulated with PHA). The proliferation of lymphocytes was examined by MTS assay, and the inhibition rate was calculated. RESULTS: In both culture systems, UCBMSCs could inhibit the proliferation of peripheral blood lymphocytes in a dose-dependent manner (P<0.05). But when the proportion of UCBMSCs to lymphocytes of the two systems was the same, the inhibitory effect of co-culture system was significantly stronger than that of Transwell(TM) system (P<0.05). CONCLUSION: UCBMSCs can inhibit the proliferation of peripheral blood lymphocytes, and the inhibitory effect of co-culture system is stronger than that of Transwell(TM) system.
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