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  • Title: A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA-AuNPs probe.
    Author: Zhu D, Yan Y, Lei P, Shen B, Cheng W, Ju H, Ding S.
    Journal: Anal Chim Acta; 2014 Oct 10; 846():44-50. PubMed ID: 25220140.
    Abstract:
    A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA-AuNPs probe. The target DNA could be specifically captured by probe 1 on the sensing interface. Then the circularization mixture was added to form a typical sandwich structure. In the presence of dNTPs and phi29 DNA polymerase, the RCA was initiated to produce micrometer-long single-strand DNA. Finally, the detection probe (DNA-AuNPs) could recognize RCA product to produce enzymatic electrochemical signal. Under optimal conditions, the calibration curve of synthetic target DNA had good linearity from 10aM to 10pM with a detection limit of 6.76aM (S/N=3). The developed method had been successfully applied to detect Salmonella as low as 6CFUmL(-1) in real milk sample. This proposed strategy showed great potential for clinical diagnosis, food safety and environmental monitoring.
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