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  • Title: The advancement of chemical cross-linking and mass spectrometry for structural proteomics: from single proteins to protein interaction networks.
    Author: Sinz A.
    Journal: Expert Rev Proteomics; 2014 Dec; 11(6):733-43. PubMed ID: 25227871.
    Abstract:
    During the last 15 years, chemical cross-linking combined with mass spectrometry (MS) and computational modeling has advanced from investigating 3D-structures of isolated proteins to deciphering protein interaction networks. In this article, the author discusses the advent, the development and the current status of the chemical cross-linking/MS strategy in the context of recent technological developments. A direct way to probe in vivo protein-protein interactions is by site-specific incorporation of genetically encoded photo-reactive amino acids or by non-directed incorporation of photo-reactive amino acids. As the chemical cross-linking/MS approach allows the capture of transient and weak interactions, it has the potential to become a routine technique for unraveling protein interaction networks in their natural cellular environment.
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