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  • Title: Direct binding of atrial natriuretic factor to adrenocortical mitochondria.
    Author: Heisler S.
    Journal: Eur J Pharmacol; 1989 Mar 21; 162(2):281-8. PubMed ID: 2524398.
    Abstract:
    Atrial natriuretic factor (ANF) is a known antagonist of adrenocortical aldosterone synthesis and secretion. Immunocytochemical and ultrastructural autoradiographic evidence suggests that ANF may bind to mitochondria of a number of target tissues including adrenal cortex. Consequently, the ability of [125I]ANF to bind directly to isolated bovine adrenocortical mitochondria was assessed. Mitochondrial-enriched subfractions of adrenocortical homogenates were prepared by differential and sucrose gradient centrifugation. Mitochondrial membranes specifically bound [125I]ANF. At 20 degrees C equilibrium was achieved between 90 and 120 min. [125I]ANF binding was inhibited in a concentration-dependent manner by unlabelled ANF (IC50 about 5 x 10(-10) M); other substances with biological actions on glomerulosa cells (arginine vasopressin, angiotensin II) did not alter [125I]ANF binding. Similarly shorter ANF fragments including ANF-(103-125), ANF-(99-109) and ANF-(111-126) had no significant competitive effect on binding of the labelled ligand. While Ca2+ and Mg2+ had little effect on ANF binding, the divalent cation Ni2+ inhibited binding of radiolabelled ANF by 90% (IC50 about 8.3 x 10(-5) M). Scatchard analysis revealed both high and low affinity binding sites for [125I]ANF with respective KDs of 4.7 +/- 7 pM and 0.3 +/- 0.02 nM and receptor densities of 1.1 +/- 0.2 and 8.6 +/- 0.1 pmol/mg protein. At 0.2 nM, Ni2+ caused a 5-fold and 100-fold decrease in high and low affinity [125I]ANF binding, respectively. The data demonstrate that ANF binds directly to mitochondria and perhaps it is at this site that the atrial peptide negatively modulates agonist-induced aldosterone biosynthesis.
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