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  • Title: [A method for determining plasmin by the rate of fibrin gel lysis].
    Author: Popova GIu, Eremeev NL, Aĭsina RB, Kazanskaia NF.
    Journal: Biull Eksp Biol Med; 1989 May; 107(5):561-4. PubMed ID: 2525408.
    Abstract:
    A simple and sensitive method has been developed to assess the fibrinolytic activity of plasmin from the change in the column height of fibrin gel. Two conditions were used: 1) 37 degrees C and 16 h incubation at plasmin concentrations of 0.5-50 micrograms/ml and 2) 25 degrees C and 1-2.5 h incubation at plasmin concentrations of 50-1000 micrograms/ml. The method permits to observe the kinetics of fibrinolysis at plasmin concentrations higher that 10 micrograms/ml. The results have shown that the method is applicable for quantitation of plasminogen in human plasma. The method is precise and well reproducible.
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