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  • Title: Production of optically pure d-lactate from glycerol by engineered Klebsiella pneumoniae strain.
    Author: Feng X, Ding Y, Xian M, Xu X, Zhang R, Zhao G.
    Journal: Bioresour Technol; 2014 Nov; 172():269-275. PubMed ID: 25270041.
    Abstract:
    In this study, glycerol was used to produce optically pure d-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, d-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve d-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in d-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1g/L optically pure d-lactate with a yield of 0.82g/g glycerol, which represented the highest d-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into d-lactate and high potentiality in utilization of crude glycerol from biodiesel industry.
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