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  • Title: Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.
    Author: Pearson CS, Belfort G, Belfort M, Shekhtman A.
    Journal: Biomol NMR Assign; 2015 Oct; 9(2):235-8. PubMed ID: 25281002.
    Abstract:
    The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.
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