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  • Title: Production of squalene by squalene synthases and their truncated mutants in Escherichia coli.
    Author: Katabami A, Li L, Iwasaki M, Furubayashi M, Saito K, Umeno D.
    Journal: J Biosci Bioeng; 2015 Feb; 119(2):165-71. PubMed ID: 25282635.
    Abstract:
    Squalene is a precursor of thousands of bioactive triterpenoids and also has industrial value as a lubricant, health-promoting agent, and/or drop-in biofuel. To establish an efficient Escherichia coli-based system for squalene production, we tested two different squalene synthases and their mutants in combination with precursor pathways. By co-expressing a chimeric mevalonate pathway with human or Thermosynechococcus squalene synthase, E. coli accumulated squalene up to 230 mg/L or 55 mg/g-DCW in flask culture. We also determined that a significant truncation of squalene synthase at the C-terminus retains partial cellular activity. The squalene-producing strain described herein represents a convenient platform for gene discovery and the construction of the pathway toward natural and non-natural hopanoids/steroids.
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