These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: N-glycosidase treatment of Colo 205 cells interferes with hIFN-gamma induced HLA-DR expression. Author: Fischer T, Wiegmann K, Pfizenmaier K. Journal: Lymphokine Res; 1989; 8(3):305-9. PubMed ID: 2528664. Abstract: We investigated the effects of N-glycosidase treatment on the interferon-gamma (IFN-gamma) induced HLA-DR expression of Colo 205 cells. N-glycosidase treatment resulted in a significant decrease of IFN-gamma induced HLA-DR specific immunofluorescence intensity ranging from complete reduction to approximately 30% of that of untreated control cells depending on the IFN-gamma dose. IFN-gamma binding studies showed that this was due to a severe reduction in IFN-gamma binding capacity of N-glycosidase treated cells. Since the number of cell membrane IFN-gamma receptors (IFN-gamma-Rs) was virtually unchanged as revealed by immunofluorescence analysis with a hIFN-gamma-R specific monoclonal antibody this indicates that N-linked carbohydrates play an important role in signal transduction and ligand binding capacity of the hIFN-gamma-R and strengthens the view that carbohydrate moieties of receptor proteins are of greater functional significance than originally anticipated.[Abstract] [Full Text] [Related] [New Search]