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  • Title: [Kinetics of specific (codon-dependent) binding of aminoacyl-tRNA to the 30S ribosomal subunit under different medium conditions].
    Author: Bogatyreva SA.
    Journal: Biokhimiia; 1978 Nov; 43(11):1973-6. PubMed ID: 252917.
    Abstract:
    It was shown that the increase of Mg2+ ions concentration in the medium from 0,01 M to 0,03 M speeds up the formation of a codon-dependent complex between 14C-Phe-tRNA and the 30S ribosomal subparticle. Under high MgCl2 concentration (0,02 M) the increase of NH4Cl concentration also accelerates the specific binding of 14C-Phe-tRNA to the 30S subparticle. In the presence of 0,005 M MgCl2 0,5 M urea significantly decreases the rate of the specific binding. 0,5 M ethanol does not have any noticeable effect on the kinetics of the reaction.
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