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  • Title: Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures.
    Author: Hong SK, Chang BK, Song SH, Kim EC.
    Journal: Indian J Med Microbiol; 2014; 32(4):419-22. PubMed ID: 25297028.
    Abstract:
    We evaluated the feasibility of same-day routine aerobic bacterial identification using the following procedures: Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The matched identification rate of this procedure at the species level was 80.6% (141/175) for the 4-h cultures compared with overnight cultures and 90.9% (159/175) for the 6-h cultures. Thus, our technique provides an easy and rapid method for identification of aerobic bacteria in routine clinical microbiology laboratories.
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