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  • Title: [Morphological observation of erythroid progenitor cell differentiation in vitro].
    Author: Zhang QY.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1989 Jun; 11(3):210-4. PubMed ID: 2529990.
    Abstract:
    Iscove's methylcellulose method was adopted and modified for the culture of erythroid progenitor cells isolated from BABL/c mouse bone marrow, and in vitro differentiation of the erythroid cell colonies was observed morphologically. Experimental results indicated: (1) in culture medium with a low concentration of erythropoietin, colonies of different sizes composed of tens to several tens of largely unsynchronized cells were formed from the late erythroid progenitor cells (CFU-E); (2) in culture medium with a high concentration of erythropoietin, the early erythroid progenitor cells (BFU-E) could be stimulated to form larger colonies which consisted of several thousand to ten thousand more highly synchronized cells; and (3) the process of pyknosis and extrusion of nuclei from the late erythroblasts could be readily observed in both the CFU-E and BFU-E colonies.
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