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  • Title: Maize (Zea mays L.).
    Author: Frame B, Warnberg K, Main M, Wang K.
    Journal: Methods Mol Biol; 2015; 1223():101-17. PubMed ID: 25300834.
    Abstract:
    Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
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