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  • Title: Pepper, chili (Capsicum annuum).
    Author: Min J, Shin SH, Jeon EM, Park JM, Hyun JY, Harn CH.
    Journal: Methods Mol Biol; 2015; 1223():311-20. PubMed ID: 25300851.
    Abstract:
    Pepper is a recalcitrant plant for Agrobacterium-mediated genetic transformation. Several obstacles to genetic transformation remain such as extremely low transformation rates; the choice of correct genotype is critical; and there is a high frequency of false positives due to direct shoot formation. Here, we report a useful protocol with a suitable selection method. The most important aspect of the pepper transformation protocol is selecting shoots growing from the callus, which is referred to as callus-mediated shoot formation. This protocol is a reproducible and reliable system for pepper transformation.
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