These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effects of (-)15-deoxyspergualin on pancreatic islet B-cell function in vitro and on the development of diabetes after multiple low dose streptozotocin administration.
    Author: Strandell E, Andersson A, Groth CG, Sandler S.
    Journal: Pharmacol Toxicol; 1989 Aug; 65(2):114-8. PubMed ID: 2530505.
    Abstract:
    The effects of (-)15-deoxyspergualin (15-DS), a newly described immunosuppressive drug, have been investigated on diabetes induced in mice treated with multiple low-dose streptozotocin (multiple SZ). Male C3D2F1 mice were treated with either intraperitoneal injections of saline or 15-DS (2.5 mg/kg body weight) for a total of 10 days, starting during the first day of SZ administration (5 days; 40 mg/kg body weight). On day 14, 15-DS-treated animals were still normoglycaemic, whilst on day 21 there was only a partial reduction in the hyperglycaemia compared to that found in the saline treated animals receiving SZ. 15-DS did not prevent hyperglycaemia in the long run (day 35-63). Furthermore, morphological examinations of pancreatic glands suggested that the insulitis in the pancreatic islets was delayed in the 15-DS-treated animals. In control experiments mice were treated with 15-DS+ the vehicle for SZ. This regimen did not hamper the glucose homeostasis of the animals. In vitro effects of 15-DS were also examined. Isolated islets from C3D2F1 mice were cultured at different concentrations of 15-DS (0.1-10.0 mg/l). After one week in culture, islet insulin release, islet insulin and DNA content were measured. The islets looked fluffy after culture at the higher 15-DS concentrations (4.0-10.0 mg/l) and at 10 mM almost all of the islets disappeared. A dose-dependent reduction of glucose-stimulated insulin release could also be seen. In other experiments islets were exposed to SZ and subsequently cultured in the presence of 0.5 mM 15-DS, however, 15-DS could not prevent the reduction in insulin release due to SZ exposure. Since 15-DS influences macrophage functions, the presently observed protective effects against the multiple SZ-treatment could reflect a reduced local interleukin-1 production in the islet vicinity. Alternatively, a lowered interleukin-1 secretion could prevent the activation of other immune cells involved in the destruction of B-cells.
    [Abstract] [Full Text] [Related] [New Search]