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  • Title: Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.
    Author: Tomavo S, Schwarz RT, Dubremetz JF.
    Journal: Mol Cell Biol; 1989 Oct; 9(10):4576-80. PubMed ID: 2531282.
    Abstract:
    The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.
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