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  • Title: Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices.
    Author: Lauer ME, Loftis J, de la Motte C, Hascall VC.
    Journal: Methods Mol Biol; 2015; 1229():543-8. PubMed ID: 25325979.
    Abstract:
    During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.
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