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  • Title: Metabolomics profile comparisons of irradiated and nonirradiated stored donor red blood cells.
    Author: Patel RM, Roback JD, Uppal K, Yu T, Jones DP, Josephson CD.
    Journal: Transfusion; 2015 Mar; 55(3):544-52. PubMed ID: 25330719.
    Abstract:
    BACKGROUND: Understanding the metabolites that are altered by donor red blood cell (RBC) storage and irradiation may provide insight into the metabolic pathways disrupted by the RBC storage lesion. STUDY DESIGN AND METHODS: Patterns of metabolites, representing more than 11,000 distinct mass-to-charge ratio (m/z) features, were compared between gamma-irradiated and nonirradiated CPDA-1-split RBCs from six human donors over 35 days of storage using multilevel sparse partial least squares discriminant analysis (msPLSDA), hierarchical clustering, pathway enrichment analysis, and network analysis. RESULTS: In msPLSDA analysis, RBC units stored 7 days or fewer (irradiated or nonirradiated) showed similar metabolomic profiles. By contrast, donor RBCs stored 10 days or more demonstrated distinct clustering as a function of storage time and irradiation. Irradiation shifted metabolic features to those seen in older units. Hierarchical clustering analysis identified at least two clusters of metabolites that differentiated between RBC units based on storage time and irradiation exposure, confirming results of the msPLSDA analysis. Pathway enrichment analysis, used to map the discriminatory biochemical features to specific metabolic pathways, identified four pathways significantly affected by irradiation and/or storage including arachidonic acid (p = 3.3 × 10(-33)) and linoleic acid (p = 1.61 × 10(-11)) metabolism. CONCLUSION: RBC storage under blood bank conditions produces numerous metabolic alterations. Gamma irradiation accentuates these differences as the age of blood increases, indicating that at the biochemical level irradiation accelerates metabolic aging of stored RBCs. Metabolites involved in the cellular membrane are prominently affected and may be useful biomarkers of the RBC storage lesion.
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