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  • Title: X-ray diffraction of lipid model membranes.
    Author: Tyler AI, Law RV, Seddon JM.
    Journal: Methods Mol Biol; 2015; 1232():199-225. PubMed ID: 25331138.
    Abstract:
    In this chapter the use of X-ray diffraction to study the structure of lyotropic phases and lipid model membranes is described. Determination of the phase symmetry and lattice parameters from small-angle X-ray scattering (SAXS), and of the nature of the hydrocarbon chain packing from wide-angle X-ray scattering (WAXS), are discussed. Methods by which the sign of the interfacial curvature of non-lamellar phases may be determined are then presented. Finally, the calculation of electron density profiles from the intensities of the observed Bragg peaks is described, for the lamellar phase and for the inverse hexagonal phase.
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