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  • Title: Molecular cloning, sequence analysis and mRNA expression of human ADP-ribosylation factor.
    Author: Peng ZG, Calvert I, Clark J, Helman L, Kahn R, Kung HF.
    Journal: Biofactors; 1989 Mar; 2(1):45-9. PubMed ID: 2535313.
    Abstract:
    The ADP-ribosylation factor (ARF) is the small (21 kb) GTP-binding protein required for the efficient cholera toxin-catalyzed ADP-ribosylation of purified Gs, the stimulating regulatory component of adenylate cyclase. Human ARF cDNA clones were obtained from a human cDNA library by cross-species hybridization with bovine ARF1, and the nucleotide and deduced amino acid sequences were determined. Comparison of the sequences of human and bovine ARF1 showed 90% identity at the nucleotide level and 100% identity at the amino acid level, demonstrating the highly conserved nature of the ARF protein. Using human ARF cDNA as the probe, we have detected ARF messenger RNA (approximately 2.2-2.3 kb) in a wide variety of human tissues and tumor cell lines.
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