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  • Title: Localization and characterization of alpha-2 adrenoceptors in the isolated canine pulmonary vein.
    Author: Ohlstein EH, Horohonich S, Shebuski RJ, Ruffolo RR.
    Journal: J Pharmacol Exp Ther; 1989 Jan; 248(1):233-9. PubMed ID: 2536428.
    Abstract:
    Alpha-1 and alpha-2 adrenoceptor-mediated contractile responses were studied in isolated canine pulmonary veins. Norepinephrine elicited concentration-dependent contractile responses which were antagonized in a competitive manner by the selective alpha-2 adrenergic antagonist, rauwolscine, with a dissociation constant of 15.7 nM, whereas the selective alpha-1 adrenoceptor antagonist, prazosin, produced nonparallel rightward shifts in the norepinephrine concentration-response curve with a marked depression in the maximal response, indicating noncompetitive antagonism. B-HT 933, a selective alpha-2 adrenoceptor agonist, also produced a concentration-dependent contraction in canine pulmonary vein, with the maximal contraction elicited by B-HT 933 being approximately 45% of that produced by norepinephrine. The response mediated by B-HT 933 was antagonized in a competitive manner by rauwolscine with a dissociation constant of 4.4 nM, whereas prazosin again behaved in a noncompetitive manner producing nonparallel rightward shifts in the B-HT 933 concentration-response curve with a marked depression in the maximal response. However, another alpha-1 adrenoceptor antagonist, corynanthine, weakly blocked the response produced by B-HT 933 with a dissociation constant of 1400 nM, and this low affinity for corynanthine is consistent with interaction at alpha-2 adrenoceptors. Cirazoline, a selective alpha-1 adrenoceptor agonist, also produced a concentration-dependent vasoconstrictor response in canine pulmonary veins which was antagonized competitively by both alpha-1 adrenoceptor antagonists, corynanthine and prazosin, with dissociation constants of 180 and 1.4 nM, respectively, indicative of an interaction with alpha-1 adrenoceptors. Rauwolscine (10 nM) did not significantly affect the response produced by cirazoline.(ABSTRACT TRUNCATED AT 250 WORDS)
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