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  • Title: [Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface].
    Author: Presnova GV, Rubtsova MY, Presnov DE, Grigorenko VG, Yaminsky IV, Egorov AM.
    Journal: Biomed Khim; 2014; 60(5):538-42. PubMed ID: 25386881.
    Abstract:
    The potential of the method of scanning electron microscopy (SEM) to visualize the results of individual acts of DNA and oligonucleotides hybridization using gold nanoparticles as label was investigated. Molecule of biotin was introduced into DNA or oligonucleotide, and then it was detected in DNA duplex using a conjugate of streptavidin with gold nanoparticles. Effective imaging of DNA duplexes was possible using a conjugate prepared by covalent binding.. The detection limit of the model oligonucleotide of 19 bases was 20 pg. Provedeno issledovanie vozmozhnosteĭ metoda skaniruiushcheĭ élektronnoĭ mikroskopii dlia vizualizatsii rezul'tatov edinichnykh aktov gibridizatsii DNK i oligonukleotidnykh zondov s ispol'zovaniem nanochastits zolota v kachestve metki. V issleduemuiu DNK ili oligonukleotid vvodili molekulu biotina, kotoruiu zatem vyiavliali v dupleksakh DNK s ispol'zovaniem kon"iugata streptavidina s nanochastitsami zolota. Pokazano, chto éffektivnaia vizualizatsiia dupleksov DNK vozmozhna s ispol'zovaniem kon"iugata, poluchennogo metodom kovalentnogo sviazyvaniia. Predel obnaruzheniia model'nogo oligonukleotida iz 19 osnovaniĭ sostavil 20 pg.
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