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Title: Ethylene-producing bacteria that ripen fruit. Author: Digiacomo F, Girelli G, Aor B, Marchioretti C, Pedrotti M, Perli T, Tonon E, Valentini V, Avi D, Ferrentino G, Dorigato A, Torre P, Jousson O, Mansy SS, Del Bianco C. Journal: ACS Synth Biol; 2014 Dec 19; 3(12):935-8. PubMed ID: 25393892. Abstract: Ethylene is a plant hormone widely used to ripen fruit. However, the synthesis, handling, and storage of ethylene are environmentally harmful and dangerous. We engineered E. coli to produce ethylene through the activity of the ethylene-forming enzyme (EFE) from Pseudomonas syringae. EFE converts a citric acid cycle intermediate, 2-oxoglutarate, to ethylene in a single step. The production of ethylene was placed under the control of arabinose and blue light responsive regulatory systems. The resulting bacteria were capable of accelerating the ripening of tomatoes, kiwifruit, and apples.[Abstract] [Full Text] [Related] [New Search]