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  • Title: Performance testing of a semi-automatic card punch system, using direct STR profiling of DNA from blood samples on FTA™ cards.
    Author: Ogden SJ, Horton JK, Stubbs SL, Tatnell PJ.
    Journal: J Forensic Sci; 2015 Jan; 60 Suppl 1():S207-12. PubMed ID: 25407399.
    Abstract:
    The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA(™) sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch.
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