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  • Title: DCCD sensitivity of electron and proton transfer by NADH: ubiquinone oxidoreductase in bovine heart submitochondrial particles--a thermodynamic approach.
    Author: Vuokila PT, Hassinen IE.
    Journal: Biochim Biophys Acta; 1989 May 08; 974(2):219-22. PubMed ID: 2540836.
    Abstract:
    The sensitivity of the H+/2e- ratio of the redox-driven proton pumping by the NADH: ubiquinone reductase (complex I) of the submitochondrial particles to dicyclohexylcarbodiimide (DCCD) was studied by a thermodynamic approach, measuring the membrane potential and delta pH across the membrane and the redox potential difference across the complex I span of the respiratory chain. The delta Gr/delta muH+ ratio did not decrease upon additions of 50 or 100 nmol of DCCD per mg protein in the presence of oligomycin although the H+/2e- ratio has been demonstrated to decrease upon DCCD addition in kinetic experiments with mitochondria. Complex I then becomes reminiscent of the cytochrome bc1 complex, which shows DCCD sensitivity of the kinetically but not thermodynamically determined H+/2e- ratio.
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