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  • Title: A unique type of pyrrole-based cyanine fluorophores with turn-on and ratiometric fluorescence signals at different pH regions for sensing pH in enzymes and living cells.
    Author: He L, Lin W, Xu Q, Wei H.
    Journal: ACS Appl Mater Interfaces; 2014 Dec 24; 6(24):22326-33. PubMed ID: 25408468.
    Abstract:
    The development of new functional fluorescent dyes has attracted great attention. Herein we have described a novel strategy to design a unique type of cyanine dyes by attaching two indolium moieties at the α-positions of the pyrrole core. The new type of cyanine dyes is named as PyCy fluorophores. Importantly, PyCy dyes can exhibit an exceptional feature, fluorescence turn-on response at pH varying from acidic to near-neutral conditions, and a ratiometric fluorescence response at pH varying from near-neutral to basic conditions. By taking advantage of the fluorescence turn-on response of PyCy2 at pH varying from acidic to near-neutral conditions and emission properties of PyCy2, we have demonstrated that a small-molecule fluorescent probe can image pH variations in living cells. Furthermore, we have demonstrated that PyCy2 can sense real-time pH changes under alkaline conditions induced by enzymes based on the ratiometric fluorescence response of PyCy2 at pH varying from near-neutral to basic conditions. We expect that the new design strategy for PyCy fluorophores may prompt the development of a wide variety of cyanine derivatives with desirable properties.
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