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Title: Pineapple [Ananas comosus (L.) Merr]. Author: Gangopadhyay G, Mukherjee KK. Journal: Methods Mol Biol; 2015; 1224():293-305. PubMed ID: 25416266. Abstract: The efficacy of Agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. The detailed protocol using a standard plant transformation vector (pCAMBIA1304) as reported in an 'elite' Indian variety (Queen) of pineapple [Ananas comosus (L.) Merr] can be applied to other varieties of pineapple for introgression of target genes.[Abstract] [Full Text] [Related] [New Search]