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  • Title: Cytochrome oxidase of an acidophilic iron-oxidizing bacterium, Thiobacillus ferrooxidans, functions at pH 3.5.
    Author: Kai M, Yano T, Fukumori Y, Yamanaka T.
    Journal: Biochem Biophys Res Commun; 1989 Apr 28; 160(2):839-43. PubMed ID: 2541715.
    Abstract:
    Cytochrome oxidase of Thiobacillus ferrooxidans was partially purified. The oxidase preparation had haems a and c, and oxidized ferrocytochrome c-552 of the bacterium. The optimal pH of the reaction was 3.5. The enzyme also oxidized the reduced form of rusticyanin, a copper protein of the bacterium. Our results indicate that the reduction of molecular oxygen by this enzyme may occur in the periplasm.
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