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  • Title: Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone.
    Author: Amadori A, Ciminale V, Calabro ML, Tessarollo L, Francavilla E, Chieco-Bianchi L.
    Journal: AIDS Res Hum Retroviruses; 1989 Feb; 5(1):73-8. PubMed ID: 2541750.
    Abstract:
    An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.
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