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  • Title: Next-generation sequencing of the yellowfin tuna mitochondrial genome reveals novel phylogenetic relationships within the genus Thunnus.
    Author: Guo L, Li M, Zhang H, Yang S, Chen X, Meng Z, Lin H.
    Journal: Mitochondrial DNA A DNA Mapp Seq Anal; 2016 May; 27(3):2089-90. PubMed ID: 25418629.
    Abstract:
    Recently, the next-generation sequencing (NGS) technology has become a powerful tool for sequencing the teleost mitochondrial genome (mitogenome). Here, we used this technology to determine the mitogenome of the yellowfin tuna (Thunnus albacares). A total of 41,378 reads were generated by Illumina platform with an average depth of 250×. The mitogenome (16,528 bp in length) contained 37 mitochondrial genes with the similar gene order to other typical teleosts. These mitochondrial genes were encoded on the heavy strand except for ND6 and eight tRNA genes. The result of phylogenetic analysis supported two distinct clades dividing the genus Thunnus, but the tuna species of these two genetic clades were different from that of two recognized subgenus based on anatomical characters and geographical distribution. Our results might help to understand the structure, function, and evolutionary history of the yellowfin tuna mitogenome and also provide valuable new insights for phylogenetic affinity of tuna species.
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