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  • Title: Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+-ATPase mRNA.
    Author: Lompre AM, de la Bastie D, Boheler KR, Schwartz K.
    Journal: FEBS Lett; 1989 May 22; 249(1):35-41. PubMed ID: 2542094.
    Abstract:
    Sarcoplasmic reticulum Ca2+-ATPase cDNA clones have been isolated from an adult rat heart cDNA library and the nucleotide sequence of the Ca2+-ATPase mRNA determined. The sequence has an open reading frame of 997 codons. It is identical to a cDNA isolated from a rat stomach cDNA library and 90% isologous to the rabbit and human slow/cardiac cDNAs. Nuclease S1 mapping analysis indicates that this sequence corresponds to the main Ca2+-ATPase mRNA present in heart and in slow skeletal muscle and that it is expressed in various proportions in smooth and non-muscle tissues, together with another isoform which differs from the cardiac form in the sequence of its 3'-end.
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