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  • Title: Cytoplasmic pH in cultured porcine thyroid cells: alkalinization by epidermal growth factor.
    Author: Takasu N, Nagasawa Y, Komiya I, Shimizu Y, Yamada T.
    Journal: Biochim Biophys Acta; 1989 Jun 15; 1012(1):117-20. PubMed ID: 2543453.
    Abstract:
    We studied the effects of epidermal growth factor (EGF), thyroid-stimulating hormone (TSH) and amiloride on cytoplasmic pH (pHi) in cultured porcine thyroid cells. We used 2',7'-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein (BCECF), an internalized fluorescent pH indicator, to measure pHi. EGF stimulated thyroid cell alkalinization and proliferation, which were blocked by amiloride. EGF-stimulated thyroid cell alkalinization depended on extracellular Na+ concentrations. EGF stimulation resulted in an activation of Na+/H+ exchange, which alkalinized the cells. The results indicated that Na+/H+ exchange or cell alkalinization might function as a transmembrane signal transducer in the action of EGF. In the present system, TSH did not stimulate alkalinization or proliferation.
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