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  • Title: Disposable and reliable electrochemical magnetoimmunosensor for Fumonisins simplified determination in maize-based foodstuffs.
    Author: Jodra A, López MÁ, Escarpa A.
    Journal: Biosens Bioelectron; 2015 Feb 15; 64():633-8. PubMed ID: 25441412.
    Abstract:
    An electrochemical magnetoimmunosensor involving magnetic beads and disposable carbon screen-printed electrode (CSPE) for Fumonosins (FB1, FB2 and FB3) has been developed and evaluated through a certified reference material (CRM) and beer samples. Once the immunochemical reactions took place on the magnetic beads solution, they were confined on the surface of CSPE, where electrochemical detection is achieved through the addition of suitable substrate and mediator for enzymatic tracer (Horseradish peroxidase--HRP). A remarkable detection limit of 0.33 μg L(-1), outstanding repeatability and reproducibility (RSD(intraday) of 5.6% and 2.9%; RSD(interday) of 6.9% and 6.0%; both for 0 and 5 μg L(-1) FB1 respectively), and excellent accuracy with recovery rate of 85-96% showed the suggested approach to be a very suitable screening tool for the analysis of Fumonisin B1 and B2 in food samples. A simultaneous simplified calibration and analysis protocol allows a fast and reliable determination of Fumonisin in beer samples with recovery rate of 87-105%. This strategy enhanced the analytical merits of immunosensor approach towards truly disposable tools for food-safety monitoring.
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