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  • Title: Detection of beta-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique.
    Author: Winichagoon P, Kownkon J, Yenchitsomanus P, Thonglairoam V, Siritanaratkul N, Fucharoen S.
    Journal: Hum Genet; 1989 Jul; 82(4):389-90. PubMed ID: 2544510.
    Abstract:
    Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing beta-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG----TAG) and Hb E (GAG----AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively.
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